• Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LYAR antibody (10-160) (STJ11100776)

SKU:
STJ11100776

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LYAR (10-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LYAR
Gene ID: 55646
Uniprot ID: LYAR_HUMAN
Immunogen Region: 10-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-160 of human LYAR (NP_060286.1).
Immunogen Sequence: GESVKKIQVEKHVSVCRNCE CLSCIDCGKDFWGDDYKNHV KCISEDQKYGGKGYEGKTHK GDIKQQAWIQKISELIKRPN VSPKVRELLEQISAFDNVPR KKAKFQNWMKNSLKVHNESI LDQVWNIFSEASNSEPVNKE QDQRPLHPVAN
Tissue Specificity Predominantly expressed in testis.
Function Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal.
Protein Name Cell Growth-Regulating Nucleolar Protein
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Cell Projection
Cilium
Photoreceptor Outer Segment
Component Of Pre-Ribosomal Particles
Including Pre-40s
Pre-60s And Pre-90s
Associated With Cytoplasmic Ribosomes
But Not Polysomes
As A Component Of The 60s Subunit
In The Retina
Predominantly Expressed In Photoreceptor Outer Segments
In The Nucleolus
Colocalizes With Nucleolin/Ncl
Therefore May Reside In The Dense Fibrillar Component (Dfc)
Alternative Antibody Names Anti-Cell Growth-Regulating Nucleolar Protein antibody
Anti-LYAR antibody
Anti-PNAS-5 antibody

Information sourced from Uniprot.org

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