![Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89712/358194/STJ11100776_1__10553.1713122510.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89712/358194/STJ11100776_1__10553.1713122510.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-LYAR antibody (10-160) (STJ11100776)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal LYAR (10-160) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LYAR |
| Gene ID | 55646 |
| Uniprot ID | LYAR_HUMAN |
| Immunogen Region | 10-160 |
| Immunogen Sequence | GESVKKIQVEKHVSVCRNCE CLSCIDCGKDFWGDDYKNHV KCISEDQKYGGKGYEGKTHK GDIKQQAWIQKISELIKRPN VSPKVRELLEQISAFDNVPR KKAKFQNWMKNSLKVHNESI LDQVWNIFSEASNSEPVNKE QDQRPLHPVAN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 10-160 of human LYAR (NP_060286.1). |
Additional Info
| Tissue Specificity | Predominantly expressed in testis. |
| Function | Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal. |
| Protein Name | Cell Growth-Regulating Nucleolar Protein |
| Database Links | |
| Cellular Localisation | NucleusNucleolusCytoplasmCell ProjectionCiliumPhotoreceptor Outer SegmentComponent Of Pre-Ribosomal ParticlesIncluding Pre-40sPre-60s And Pre-90sAssociated With Cytoplasmic RibosomesBut Not PolysomesAs A Component Of The 60s SubunitIn The RetinaPredominantly Expressed In Photoreceptor Outer SegmentsIn The NucleolusColocalizes With Nucleolin/NclTherefore May Reside In The Dense Fibrillar Component (Dfc) |
| Alternative Antibody Names | Anti-Cell Growth-Regulating Nucleolar Protein antibodyAnti-LYAR antibodyAnti-PNAS-5 antibody |
Information sourced from Uniprot.org