| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Volume-regulated anion channel subunit LRRC8A is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LRRC8A |
| Gene ID: |
56262 |
| Uniprot ID: |
LRC8A_HUMAN |
| Specificity: |
This antibody detects endogenous levels of LRRC8A/SWELL1 at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human LRRC8A/SWELL1 |
| Post Translational Modifications | N-glycosylated. |
| Function | Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. LRRC8A and LRRC8D are required for the uptake of the drug cisplatin. In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol: mediates both import and export of 2'-3'-cGAMP, thereby promoting transfer of 2'-3'-cGAMP to bystander cells. In contrast, complexes containing LRRC8D inhibit transport of 2'-3'-cGAMP. Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E).channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition. Also required for T-cell development. Required for myoblast differentiation: VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption. Also acts as a regulator of glucose-sensing in pancreatic beta cells: VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion. Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition: lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis. |
| Protein Name | Volume-Regulated Anion Channel Subunit Lrrc8aLeucine-Rich Repeat-Containing Protein 8aHslrrc8aSwelling Protein 1 |
| Database Links | Reactome: R-HSA-5223345 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinLysosome MembraneMainly Localizes To The Cell MembraneWith Some Intracellular Localization To Lysosomes |
| Alternative Antibody Names | Anti-Volume-Regulated Anion Channel Subunit Lrrc8a antibodyAnti-Leucine-Rich Repeat-Containing Protein 8a antibodyAnti-Hslrrc8a antibodyAnti-Swelling Protein 1 antibodyAnti-LRRC8A antibodyAnti-KIAA1437 antibodyAnti-LRRC8 antibodyAnti-SWELL1 antibodyAnti-UNQ221 antibodyAnti-PRO247 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
