Anti-LRRC8A antibody (STJA0008517)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	LRRC8A
Gene ID:	56262
Uniprot ID:	LRC8A_HUMAN
Specificity:	This antibody detects endogenous levels of LRRC8A/SWELL1 at Human, Mouse, Rat
Immunogen:	Synthesized peptide derived from human LRRC8A/SWELL1

Post Translational Modifications	N-glycosylated.
Function	Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. LRRC8A and LRRC8D are required for the uptake of the drug cisplatin. In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol: mediates both import and export of 2'-3'-cGAMP, thereby promoting transfer of 2'-3'-cGAMP to bystander cells. In contrast, complexes containing LRRC8D inhibit transport of 2'-3'-cGAMP. Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E).channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition. Also required for T-cell development. Required for myoblast differentiation: VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption. Also acts as a regulator of glucose-sensing in pancreatic beta cells: VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion. Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition: lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis.
Protein Name	Volume-Regulated Anion Channel Subunit Lrrc8a Leucine-Rich Repeat-Containing Protein 8a Hslrrc8a Swelling Protein 1
Database Links	Reactome: R-HSA-5223345
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Lysosome Membrane Mainly Localizes To The Cell Membrane With Some Intracellular Localization To Lysosomes
Alternative Antibody Names	Anti-Volume-Regulated Anion Channel Subunit Lrrc8a antibody Anti-Leucine-Rich Repeat-Containing Protein 8a antibody Anti-Hslrrc8a antibody Anti-Swelling Protein 1 antibody Anti-LRRC8A antibody Anti-KIAA1437 antibody Anti-LRRC8 antibody Anti-SWELL1 antibody Anti-UNQ221 antibody Anti-PRO247 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance