• Immunofluorescence analysis of NIH/3T3 cells using LPP Rabbit monoclonal antibody (STJ11102958) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using LPP Rabbit monoclonal antibody (STJ11102958) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using LPP antibody (STJ11102958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LPP antibody (1-100) [S8MR] (STJ11102958)

SKU:
STJ11102958

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LPP (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LPP
Gene ID: 4026
Uniprot ID: LPP_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LPP (Q93052).
Immunogen Sequence: MSHPSWLPPKSTGEPLGHVP ARMETTHSFGNPSISVSTQQ PPKKFAPVVAPKPKYNPYKQ PGGEGDFLPPPPPPLDDSSA LPSISGNFPPPPPLDEEAFK
Tissue Specificity Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes.
Function May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.
Protein Name Lipoma-Preferred Partner
Lim Domain-Containing Preferred Translocation Partner In Lipoma
Cellular Localisation Nucleus
Cytoplasm
Cell Junction
Cell Membrane
Found In The Nucleus
In The Cytoplasm And At Cell Adhesion Sites
Shuttles Between The Cytoplasm And The Nucleus
It Has Been Found In Sites Of Cell Adhesion Such As Cell-To-Cell Contact And Focal Adhesion Which Are Membrane Attachment Sites Of Cells To The Extracellular Matrix
Mainly Nuclear When Fused With Hmga2/Hmgic And Kmt2a/Mll1
Alternative Antibody Names Anti-Lipoma-Preferred Partner antibody
Anti-Lim Domain-Containing Preferred Translocation Partner In Lipoma antibody
Anti-LPP antibody

Information sourced from Uniprot.org

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