• Western blot analysis of extracts of various cell lines, using Lamin B2 rabbit monoclonal antibody (STJ11102218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Lamin B2 rabbit monoclonal antibody (STJ11102218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Lamin B2 rabbit monoclonal antibody (STJ11102218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Lamin B2 rabbit monoclonal antibody (STJ11102218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded human colon carcinoma tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded human spleen tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded mouse brain tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded mouse kidney tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded mouse liver tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Confocal imaging of HeLa cells using Lamin B2 rabbit monoclonal antibody (STJ11102218, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin rabbit monoclonal antibody (dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-LMNB2 antibody (521-620) [S8MR] (STJ11102218)

SKU:
STJ11102218

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Lamin B2 (521-620) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB2
Gene ID: 84823
Uniprot ID: LMNB2_HUMAN
Immunogen Region: 521-620
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 521-620 of human Lamin B2 (Q03252).
Immunogen Sequence: YILRAGQMVTVWAAGAGVAH SPPSTLVWKGQSSWGTGESF RTVLVNADGEEVAMRTVKKS SVMRENENGEEEEEEAEFGE EDLFHQQGDPRTTSRGCYVM
Post Translational Modifications B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Protein Name Lamin-B2
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B2 antibody
Anti-LMNB2 antibody
Anti-LMN2 antibody

Information sourced from Uniprot.org

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