• Western blot analysis of extracts of various cell lines, using LGR6 rabbit monoclonal antibody (STJ11102429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of HeLa cells using LGR6 Rabbit monoclonal antibody (STJ11102429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat testis, using LGR6 rabbit monoclonal antibody (STJ11102429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Rat testis, using LGR6 Rabbit monoclonal antibody (STJ11102429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using LGR6 rabbit monoclonal antibody (STJ11102429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using LGR6 Rabbit monoclonal antibody (STJ11102429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-LGR6 antibody (801-900) [S9MR] (STJ11102429)

SKU:
STJ11102429

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GPCR/LGR6 (801-900) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LGR6
Gene ID: 59352
Uniprot ID: LGR6_HUMAN
Immunogen Region: 801-900
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 801-900 of human LGR6 (Q9HBX8).
Immunogen Sequence: FPVTPEAVKSVLLVVLPLPA CLNPLLYLLFNPHFRDDLRR LRPRAGDSGPLAYAAAGELE KSSCDSTQALVAFSDVDLIL EASEAGRPPGLETYGFPSVT
Function Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor.
Protein Name Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 6
Database Links Reactome: R-HSA-4641263
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 6 antibody
Anti-LGR6 antibody
Anti-UNQ6427 antibody
Anti-PRO21331 antibody
Anti-VTS20631 antibody

Information sourced from Uniprot.org

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