• Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L-929 cells using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and Galectin 3/Galectin 3/LGALS3 knockout (KO) HeLa cells, using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LGALS3 antibody (1-250) (STJ115467)

SKU:
STJ115467

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LGALS3 (1-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LGALS3
Gene ID: 3958
Uniprot ID: LEG3_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Galectin 3/Galectin 3/LGALS3 (NP_002297.2).
Immunogen Sequence: MADNFSLHDALSGSGNPNPQ GWPGAWGNQPAGAGGYPGAS YPGAYPGQAPPGAYPGQAPP GAYPGAPGAYPGAPAPGVYP GPPSGPGAYPSSGQPSATGA YPATGPYGAPAGPLIVPYNL PLPGGVVPRMLITILGTVKP NANRIALDFQRGNDVAFHFN PRFNENNRRVIVCNTKLDNN WGREERQSVFPFESGKPFKI QVLVEPDHFKVAVNDAHLLQ YNHRVKKLNEISKLGISGD
Tissue Specificity A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Protein Name Galectin-3
Gal-3
35 Kda Lectin
Carbohydrate-Binding Protein 35
Cbp 35
Galactose-Specific Lectin 3
Galactoside-Binding Protein
Galbp
Ige-Binding Protein
L-31
Laminin-Binding Protein
Lectin L-29
Mac-2 Antigen
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-879415
Reactome: R-HSA-8939246
Reactome: R-HSA-8941333
Cellular Localisation Cytoplasm
Nucleus
Secreted
Secreted By A Non-Classical Secretory Pathway And Associates With The Cell Surface
Can Be Secreted
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative Antibody Names Anti-Galectin-3 antibody
Anti-Gal-3 antibody
Anti-35 Kda Lectin antibody
Anti-Carbohydrate-Binding Protein 35 antibody
Anti-Cbp 35 antibody
Anti-Galactose-Specific Lectin 3 antibody
Anti-Galactoside-Binding Protein antibody
Anti-Galbp antibody
Anti-Ige-Binding Protein antibody
Anti-L-31 antibody
Anti-Laminin-Binding Protein antibody
Anti-Lectin L-29 antibody
Anti-Mac-2 Antigen antibody
Anti-LGALS3 antibody
Anti-MAC2 antibody

Information sourced from Uniprot.org

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