• Western blot analysis of various lysates using LEFTY1 Rabbit polyclonal antibody (STJ117584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LEFTY1 antibody (77-366) (STJ117584)

SKU:
STJ117584

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LEFTY1 (77-366) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LEFTY1
Gene ID: 10637
Uniprot ID: LFTY1_HUMAN
Immunogen Region: 77-366
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 77-366 of human LEFTY1 (NP_066277.1).
Immunogen Sequence: RFSQSFREVAGRFLALEAST HLLVFGMEQRLPPNSELVQA VLRLFQEPVPKAALHRHGRL SPRSARARVTVEWLRVRDDG SNRTSLIDSRLVSVHESGWK AFDVTEAVNFWQQLSRPRQP LLLQVSVQREHLGPLASGAH KLVRFASQGAPAGLGEPQLE LHTLDLGDYGAQGDCDPEAP MTEGTRCCRQEMYIDLQGMK WAENWVLEPPGFLAYECVGT CRQPPEALAFKWPFLGPRQ
Post Translational Modifications The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
Function Required for left-right axis determination as a regulator of LEFTY2 and NODAL.
Protein Name Left-Right Determination Factor 1
Left-Right Determination Factor B
Protein Lefty-1
Protein Lefty-B
Database Links Reactome: R-HSA-1181150
Reactome: R-HSA-1433617
Cellular Localisation Secreted
Alternative Antibody Names Anti-Left-Right Determination Factor 1 antibody
Anti-Left-Right Determination Factor B antibody
Anti-Protein Lefty-1 antibody
Anti-Protein Lefty-B antibody
Anti-LEFTY1 antibody
Anti-LEFTB antibody
Anti-LEFTYB antibody
Anti-UNQ278 antibody
Anti-PRO317 antibody

Information sourced from Uniprot.org

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