Anti-LDLRAP1 antibody (59-308) (STJ29173)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal LDLRAP1 (59-308) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LDLRAP1 |
| Gene ID | 26119 |
| Uniprot ID | ARH_HUMAN |
| Immunogen Region | 59-308 |
| Immunogen Sequence | EQPKGEELSAAAIKRIVATA KASGKKLQKVTLKVSPRGII LTDNLTNQLIENVSIYRISY CTADKMHDKVFAYIAQSQHN QSLECHAFLCTKRKMAQAVT LTVAQAFKVAFEFWQVSKEE KEKRDKASQEGGDVLGARQD CTPSLKSLVATGNLLDLEET AKAPLSTVSANTTNMDEVPR PQALSGSSVVWELDDGLDEA FSRLAQSRTNPQVLDTGLTA QDMHYAQCLSPVDWDKPDS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 59-308 of human LDLRAP1 (NP_056442.2). |
Additional Info
| Tissue Specificity | Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes. |
| Function | Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytosis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression. |
| Protein Name | Low Density Lipoprotein Receptor Adapter Protein 1Autosomal Recessive Hypercholesterolemia Protein |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8964026Reactome: R-HSA-8964038Reactome: R-HSA-9758890 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Low Density Lipoprotein Receptor Adapter Protein 1 antibodyAnti-Autosomal Recessive Hypercholesterolemia Protein antibodyAnti-LDLRAP1 antibodyAnti-ARH antibody |
Information sourced from Uniprot.org