• Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g LDHB antibody (STJ11102267). Western blot was performed from the immunoprecipitate using LDHB antibody (STJ11102267) at a dilution of 1:1000.
  • Immunohistochemistry analysis of LDHB in paraffin-embedded human appendix using LDHB Rabbit monoclonal antibody (STJ11102267) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LDHB in paraffin-embedded rat ovary using LDHB Rabbit monoclonal antibody (STJ11102267) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using LDHB Rabbit monoclonal antibody (STJ11102267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LDHB antibody (1-100) [S7MR] (STJ11102267)

SKU:
STJ11102267

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LDHB (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHB
Gene ID: 3945
Uniprot ID: LDHB_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LDHB (P07195).
Immunogen Sequence: MATLKEKLIAPVAEEEATVP NNKITVVGVGQVGMACAISI LGKSLADELALVDVLEDKLK GEMMDLQHGSLFLQTPKIVA DKDYSVTANSKIVVVTAGVR
Tissue Specificity Predominantly expressed in aerobic tissues such as cardiac muscle.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase B Chain
Ldh-B
Ldh Heart Subunit
Ldh-H
Renal Carcinoma Antigen Ny-Ren-46
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Mitochondrion Inner Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-L-Lactate Dehydrogenase B Chain antibody
Anti-Ldh-B antibody
Anti-Ldh Heart Subunit antibody
Anti-Ldh-H antibody
Anti-Renal Carcinoma Antigen Ny-Ren-46 antibody
Anti-LDHB antibody

Information sourced from Uniprot.org

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