• Immunofluorescence analysis of L929 cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of LDHA in paraffin-embedded Mouse kidney using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LDHA antibody (1-100) (STJ11103897)

SKU:
STJ11103897

€161.88 - €799.38
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-L-Lactate Dehydrogenase A Chain (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHA
Gene ID: 3939
Uniprot ID: LDHA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LDHA (NP_001158886.1).
Immunogen Sequence: MATLKDQLIYNLLKEEQTPQ NKITVVGVGAVGMACAISIL MKDLADELALVDVIEDKLKG EMMDLQHGSLFLRTPKIVSG KDYNVTANSKLVIITAGARQ
Tissue Specificity Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Post Translational Modifications ISGylated.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase A Chain
Ldh-A
Cell Proliferation-Inducing Gene 19 Protein
Ldh Muscle Subunit
Ldh-M
Renal Carcinoma Antigen Ny-Ren-59
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-L-Lactate Dehydrogenase A Chain antibody
Anti-Ldh-A antibody
Anti-Cell Proliferation-Inducing Gene 19 Protein antibody
Anti-Ldh Muscle Subunit antibody
Anti-Ldh-M antibody
Anti-Renal Carcinoma Antigen Ny-Ren-59 antibody
Anti-LDHA antibody
Anti-PIG19 antibody

Information sourced from Uniprot.org

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