![Immunofluorescence analysis of L929 cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176542/367362/STJ11103897_1__40674.1713132901.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of LDHA in paraffin-embedded Mouse kidney using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176542/367363/STJ11103897_2__58318.1713132901.jpg?c=1 "Immunohistochemistry analysis of LDHA in paraffin-embedded Mouse kidney using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176542/367364/STJ11103897_3__20411.1713132902.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176542/367365/STJ11103897_4__85832.1713132903.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunofluorescence analysis of L929 cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/176542/367362/STJ11103897_1__40674.1713132901.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KD Validated] LDHA Rabbit polyclonal antibody (STJ11103897) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-LDHA antibody (1-100) (STJ11103897)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal L-Lactate Dehydrogenase A Chain (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LDHA |
| Gene ID | 3939 |
| Uniprot ID | LDHA_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MATLKDQLIYNLLKEEQTPQ NKITVVGVGAVGMACAISIL MKDLADELALVDVIEDKLKG EMMDLQHGSLFLRTPKIVSG KDYNVTANSKLVIITAGARQ |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LDHA (NP_001158886.1). |
Additional Info
| Tissue Specificity | Predominantly expressed in anaerobic tissues such as skeletal muscle and liver. |
| Post Translational Modifications | ISGylated. |
| Function | Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+). |
| Protein Name | L-Lactate Dehydrogenase A ChainLdh-ACell Proliferation-Inducing Gene 19 ProteinLdh Muscle SubunitLdh-MRenal Carcinoma Antigen Ny-Ren-59 |
| Database Links | Reactome: R-HSA-70268Reactome: R-HSA-9861718 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-L-Lactate Dehydrogenase A Chain antibodyAnti-Ldh-A antibodyAnti-Cell Proliferation-Inducing Gene 19 Protein antibodyAnti-Ldh Muscle Subunit antibodyAnti-Ldh-M antibodyAnti-Renal Carcinoma Antigen Ny-Ren-59 antibodyAnti-LDHA antibodyAnti-PIG19 antibody |
Information sourced from Uniprot.org