• Western blot analysis of various lysates, using LAMP2 antibody (STJ24369) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of mouse kidney, using LAMP2 antibody (STJ24369) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using LAMP2 rabbit polyclonal antibody (STJ24369) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using LAMP2 rabbit polyclonal antibody (STJ24369) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using LAMP2 rabbit polyclonal antibody (STJ24369) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-LAMP2 antibody (29-410) (STJ24369)

SKU:
STJ24369

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LAMP2 (29-410) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:100-1:500
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LAMP2
Gene ID: 3920
Uniprot ID: LAMP2_HUMAN
Immunogen Region: 29-410
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-410 of human LAMP2 (NP_002285.1).
Immunogen Sequence: LELNLTDSENATCLYAKWQM NFTVRYETTNKTYKTVTISD HGTVTYNGSICGDDQNGPKI AVQFGPGFSWIANFTKAAST YSIDSVSFSYNTGDNTTFPD AEDKGILTVDELLAIRIPLN DLFRCNSLSTLEKNDVVQHY WDVLVQAFVQNGTVSTNEFL CDKDKTSTVAPTIHTTVPSP TTTPTPKEKPEAGTYSVNNG NDTCLLATMGLQLNITQDKV ASVININPNTTHSTGSCRS
Tissue Specificity Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.
Post Translational Modifications O- and N-glycosylated.some of the 16 N-linked glycans are polylactosaminoglycans.
Function Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH, NLRP3 and MLLT11, and targeting them for lysosomal degradation. In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2. Plays a role in lysosomal protein degradation in response to starvation. Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation.antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens. Isoform LAMP-2C: Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Protein Name Lysosome-Associated Membrane Glycoprotein 2
Lamp-2
Lysosome-Associated Membrane Protein 2
Cd107 Antigen-Like Family Member B
Lgp-96
Cd Antigen Cd107b
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-6798695
Reactome: R-HSA-9613829
Cellular Localisation Lysosome Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Cell Membrane
Cytoplasmic Vesicle
Autophagosome Membrane
This Protein Shuttles Between Lysosomes
Endosomes
And The Plasma Membrane
Alternative Antibody Names Anti-Lysosome-Associated Membrane Glycoprotein 2 antibody
Anti-Lamp-2 antibody
Anti-Lysosome-Associated Membrane Protein 2 antibody
Anti-Cd107 Antigen-Like Family Member B antibody
Anti-Lgp-96 antibody
Anti-Cd Antigen Cd107b antibody
Anti-LAMP2 antibody

Information sourced from Uniprot.org

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