• Western blot analysis of extracts from normal (control) and L1CAM knockout (KO) HeLa cells, using L1CAM antibody (STJ111291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of SH-SY5Y cells using CD171/L1CAM Rabbit polyclonal antibody (STJ111291) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using L1CAM antibody (STJ111291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of Neuro-2a cells using CD171/L1CAM Rabbit polyclonal antibody (STJ111291) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using [KO Validated] L1CAM rabbit polyclonal antibody (STJ111291) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using CD171/L1CAM Rabbit polyclonal antibody (STJ111291) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Neuro-2a cells using [KO Validated] L1CAM rabbit polyclonal antibody (STJ111291) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CD171/L1CAM antibody (STJ111291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of SH-SY5Y cells using [KO Validated] L1CAM rabbit polyclonal antibody (STJ111291) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Anti-L1CAM antibody (1143-1257) (STJ111291)

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STJ111291

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-L1CAM (1143-1257) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: L1CAM
Gene ID: 3897
Uniprot ID: L1CAM_HUMAN
Immunogen Region: 1143-1257
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human CD171/L1CAM (NP_000416.1).
Immunogen Sequence: IKRSKGGKYSVKDKEDTQVD SEARPMKDETFGEYRSLESD NEEKAFGSSQPSLNGDIKPL GSDDSLADYGGSVDVQFNED GSFIGQYSGKKEKEAAGGND SSGATSPINPAVALE
Function Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Protein Name Neural Cell Adhesion Molecule L1
N-Cam-L1
Ncam-L1
Cd Antigen Cd171
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-373760
Reactome: R-HSA-437239
Reactome: R-HSA-445095
Reactome: R-HSA-445144
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Growth Cone
Axon
Dendrite
Colocalized With Shtn1 In Close Apposition With Actin Filaments In Filopodia And Lamellipodia Of Axonalne Growth Cones Of Hippocampal Neurons
In Neurons
Detected Predominantly In Axons And Cell Body
Weak Localization To Dendrites
Alternative Antibody Names Anti-Neural Cell Adhesion Molecule L1 antibody
Anti-N-Cam-L1 antibody
Anti-Ncam-L1 antibody
Anti-Cd Antigen Cd171 antibody
Anti-L1CAM antibody
Anti-CAML1 antibody
Anti-MIC5 antibody

Information sourced from Uniprot.org

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