• Confocal imaging of human tonsil using Cytokeratin 4 (KRT4) Rabbit monoclonal antibody (STJ11101221, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Cytokeratin 4 (KRT4) (KRT4) Rabbit monoclonal antibody (STJ11101221) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cytokeratin 4 (KRT4) (KRT4) Rabbit monoclonal antibody (STJ11101221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KRT4 antibody (1-100) [S1MR] (STJ11101221)

SKU:
STJ11101221

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 4 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT4
Gene ID: 3851
Uniprot ID: K2C4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 4 (KRT4) (P19013).
Immunogen Sequence: MIARQQCVRGGPRGFSCGSA IVGGGKRGAFSSVSMSGGAG RCSSGGFGSRSLYNLRGNKS ISMSVAGSRQGACFGGAGGF GTGGFGGGFGGSFSGKGGPG
Tissue Specificity Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
Protein Name Keratin - Type Ii Cytoskeletal 4
Cytokeratin-4
Ck-4
Keratin-4
K4
Type-Ii Keratin Kb4
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 4 antibody
Anti-Cytokeratin-4 antibody
Anti-Ck-4 antibody
Anti-Keratin-4 antibody
Anti-K4 antibody
Anti-Type-Ii Keratin Kb4 antibody
Anti-KRT4 antibody
Anti-CYK4 antibody

Information sourced from Uniprot.org

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