![Western blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20) ) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ119970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98755/382808/STJ119970_1__87130.1713151301.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20) ) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ119970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20) ) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ119970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98755/382808/STJ119970_1__87130.1713151301.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Cytokeratin 20 (Cytokeratin 20 (KRT20) ) knockout (KO) HeLa cells, using [KO Validated] Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ119970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-KRT20 antibody (245-424) (STJ119970)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KRT20 (245-424) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KRT20 |
| Gene ID | 54474 |
| Uniprot ID | K1C20_HUMAN |
| Immunogen Region | 245-424 |
| Immunogen Sequence | KYEVMAQKNLQEAKEQFERQ TAVLQQQVTVNTEELKGTEV QLTELRRTSQSLEIELQSHL SMKESLEHTLEETKARYSSQ LANLQSLLSSLEAQLMQIRS NMERQNNEYHILLDIKTRLE QEIATYRRLLEGEDVKTTEY QLSTLEERDIKKTRKIKTVV QEVVDGKVVSSEVKEVEENI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 245-424 of human Cytokeratin 20 (Cytokeratin 20 (KRT20) ) (NP_061883.1). |
Additional Info
| Tissue Specificity | Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa.specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. |
| Post Translational Modifications | Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury. Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. |
| Function | Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. |
| Protein Name | Keratin - Type I Cytoskeletal 20Cytokeratin-20Ck-20Keratin-20K20Protein It |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 20 antibodyAnti-Cytokeratin-20 antibodyAnti-Ck-20 antibodyAnti-Keratin-20 antibodyAnti-K20 antibodyAnti-Protein It antibodyAnti-KRT20 antibody |
Information sourced from Uniprot.org