Anti-KRT18 antibody [DC10] (STJ180142)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenHuman breast cancer PMC 42 cells
STJ180142
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General Information

Short DescriptionMouse monoclonal KRT18 antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC-P
HostMouse
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDDC10
IsotypeIgG1k
ConjugationUnconjugated
PurificationAffinity purified
Dilution Range1:100‐200
FormulationTris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Storage InstructionStore at 2‐8Β°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24Β°C, 3 months at 2‐8Β°C, 6months at ‐20Β°C.

Target Information

Gene SymbolKRT18
Gene ID3875
Uniprot IDK1C18_HUMAN
ImmunogenHuman breast cancer PMC 42 cells
SpecificityPositive control: Breast carcinoma

Additional Info

Tissue Specificity Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Post Translational Modifications Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Function Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Protein Name Keratin - Type I Cytoskeletal 18
Cell Proliferation-Inducing Gene 46 Protein
Cytokeratin-18
Ck-18
Keratin-18
K18
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Nucleus Matrix
Cytoplasm
Perinuclear Region
Nucleus
Nucleolus
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 18 antibody
Anti-Cell Proliferation-Inducing Gene 46 Protein antibody
Anti-Cytokeratin-18 antibody
Anti-Ck-18 antibody
Anti-Keratin-18 antibody
Anti-K18 antibody
Anti-KRT18 antibody
Anti-CYK18 antibody
Anti-PIG46 antibody

Information sourced from Uniprot.org

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