• Immunofluorescence analysis of rat skin using Cytokeratin 15 (KRT15) (KRT15) Rabbit monoclonal antibody (STJ11102168) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of CAL 27 cells, using Cytokeratin 15 (KRT15) (KRT15) antibody (STJ11102168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-KRT15 antibody (357-456) [S8MR] (STJ11102168)

SKU:
STJ11102168

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 15 (357-456) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT15
Gene ID: 3866
Uniprot ID: K1C15_HUMAN
Immunogen Region: 357-456
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 357-456 of human Cytokeratin 15 (KRT15) (KRT15) (P19012).
Immunogen Sequence: YATQLQQIQGLIGGLEAQLS ELRCEMEAQNQEYKMLLDIK TRLEQEIATYRSLLEGQDAK MAGIAIREASSGGGGSSSNF HINVEESVDGQVVSSHKREI
Tissue Specificity Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.
Protein Name Keratin - Type I Cytoskeletal 15
Cytokeratin-15
Ck-15
Keratin-15
K15
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 15 antibody
Anti-Cytokeratin-15 antibody
Anti-Ck-15 antibody
Anti-Keratin-15 antibody
Anti-K15 antibody
Anti-KRT15 antibody
Anti-KRTB antibody

Information sourced from Uniprot.org

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