Anti-KMT5A antibody (282-332) (STJA0006637)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human SETD8 AA range: 282-332
General Information
| Short Description | Rabbit polyclonal anti-N-lysine methyltransferase KMT5A (282-332) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KMT5A |
| Gene ID | 387893 |
| Uniprot ID | KMT5A_HUMAN |
| Immunogen | Synthesized peptide derived from human SETD8 AA range: 282-332 |
| Immunogen Region | 282-332 |
| Specificity | This antibody detects endogenous levels of human SETD8 |
Additional Info
| Function | Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. |
| Protein Name | N-Lysine Methyltransferase Kmt5aH4-K20-Hmtase Kmt5aHistone-Lysine N-Methyltransferase Kmt5aLysine N-Methyltransferase 5aLysine-Specific Methylase 5aPr/Set Domain-Containing Protein 07Pr-Set7Pr/Set07Set Domain-Containing Protein 8 |
| Database Links | Reactome: R-HSA-2299718Reactome: R-HSA-3214841Reactome: R-HSA-6804760 |
| Cellular Localisation | NucleusChromosomeSpecifically Localizes To Mitotic ChromosomesColocalized With Sirt2 At Mitotic FociAssociates With Chromosomes During MitosisAssociation Is Increased In A H(2)O(2)-Induced Oxidative Stress-Dependent MannerAssociates With Silent Chromatin On Euchromatic ArmsNot Associated With Constitutive Heterochromatin |
| Alternative Antibody Names | Anti-N-Lysine Methyltransferase Kmt5a antibodyAnti-H4-K20-Hmtase Kmt5a antibodyAnti-Histone-Lysine N-Methyltransferase Kmt5a antibodyAnti-Lysine N-Methyltransferase 5a antibodyAnti-Lysine-Specific Methylase 5a antibodyAnti-Pr/Set Domain-Containing Protein 07 antibodyAnti-Pr-Set7 antibodyAnti-Pr/Set07 antibodyAnti-Set Domain-Containing Protein 8 antibodyAnti-KMT5A antibodyAnti-PRSET7 antibodyAnti-SET07 antibodyAnti-SET8 antibodyAnti-SETD8 antibody |
Information sourced from Uniprot.org