| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-N-lysine methyltransferase KMT5A (282-332) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | KMT5A |
| Gene ID: | 387893 |
| Uniprot ID: | KMT5A_HUMAN |
| Immunogen Region: | 282-332 |
| Specificity: | This antibody detects endogenous levels of human SETD8 |
| Immunogen: | Synthesized peptide derived from human SETD8 AA range: 282-332 |
| Function | Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. |
| Protein Name | N-Lysine Methyltransferase Kmt5a H4-K20-Hmtase Kmt5a Histone-Lysine N-Methyltransferase Kmt5a Lysine N-Methyltransferase 5a Lysine-Specific Methylase 5a Pr/Set Domain-Containing Protein 07 Pr-Set7 Pr/Set07 Set Domain-Containing Protein 8 |
| Database Links | Reactome: R-HSA-2299718 Reactome: R-HSA-3214841 Reactome: R-HSA-6804760 |
| Cellular Localisation | Nucleus Chromosome Specifically Localizes To Mitotic Chromosomes Colocalized With Sirt2 At Mitotic Foci Associates With Chromosomes During Mitosis Association Is Increased In A H(2)O(2)-Induced Oxidative Stress-Dependent Manner Associates With Silent Chromatin On Euchromatic Arms Not Associated With Constitutive Heterochromatin |
| Alternative Antibody Names | Anti-N-Lysine Methyltransferase Kmt5a antibody Anti-H4-K20-Hmtase Kmt5a antibody Anti-Histone-Lysine N-Methyltransferase Kmt5a antibody Anti-Lysine N-Methyltransferase 5a antibody Anti-Lysine-Specific Methylase 5a antibody Anti-Pr/Set Domain-Containing Protein 07 antibody Anti-Pr-Set7 antibody Anti-Pr/Set07 antibody Anti-Set Domain-Containing Protein 8 antibody Anti-KMT5A antibody Anti-PRSET7 antibody Anti-SET07 antibody Anti-SET8 antibody Anti-SETD8 antibody |
Information sourced from Uniprot.org