| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Inactive histone-lysine N-methyltransferase 2E is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KMT2E |
| Gene ID: |
55904 |
| Uniprot ID: |
KMT2E_HUMAN |
| Specificity: |
This antibody detects endogenous levels of MLL5 at Human, Mouse |
| Immunogen: |
Synthesized peptide derived from human MLL5 |
| Post Translational Modifications | Ubiquitinated. Deubiquitinated by USP7. O-glycosylated at Ser-435 and Thr-440 in the SET domain by OGT which probably prevents KMT2E proteasomal-mediated degradation. |
| Function | Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription. Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry. Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition. During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Isoform NKp44L: Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity. |
| Protein Name | Inactive Histone-Lysine N-Methyltransferase 2eInactive Lysine N-Methyltransferase 2eMyeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 5 |
| Cellular Localisation | ChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleus SpeckleAbsent From The NucleolusLocalizes To Chromosome During Interphase And To Centrosomes During MitosisDissociation From Mitotic Chromosome Is Likely Due To Histone H3 Phosphorylation On 'Thr-3' And 'Thr-6'Isoform 3: NucleusNucleoplasmLocalizes To Chromosome During Interphase And To Nucleus Speckle During MitosisIsoform Nkp44l: CytoplasmCell MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Inactive Histone-Lysine N-Methyltransferase 2e antibodyAnti-Inactive Lysine N-Methyltransferase 2e antibodyAnti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 5 antibodyAnti-KMT2E antibodyAnti-MLL5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
