Anti-KMT2E antibody (STJA0009106)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human MLL5
General Information
| Short Description | Rabbit polyclonal anti-Inactive histone-lysine N-methyltransferase 2E for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KMT2E |
| Gene ID | 55904 |
| Uniprot ID | KMT2E_HUMAN |
| Immunogen | Synthesized peptide derived from human MLL5 |
| Specificity | This antibody detects endogenous levels of MLL5 at Human, Mouse |
Additional Info
| Post Translational Modifications | Ubiquitinated. Deubiquitinated by USP7. O-glycosylated at Ser-435 and Thr-440 in the SET domain by OGT which probably prevents KMT2E proteasomal-mediated degradation. |
| Function | Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription. Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry. Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition. During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Isoform NKp44L: Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity. |
| Protein Name | Inactive Histone-Lysine N-Methyltransferase 2eInactive Lysine N-Methyltransferase 2eMyeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 5 |
| Database Links | |
| Cellular Localisation | ChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleus SpeckleAbsent From The NucleolusLocalizes To Chromosome During Interphase And To Centrosomes During MitosisDissociation From Mitotic Chromosome Is Likely Due To Histone H3 Phosphorylation On 'Thr-3' And 'Thr-6'Isoform 3: NucleusNucleoplasmLocalizes To Chromosome During Interphase And To Nucleus Speckle During MitosisIsoform Nkp44l: CytoplasmCell MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Inactive Histone-Lysine N-Methyltransferase 2e antibodyAnti-Inactive Lysine N-Methyltransferase 2e antibodyAnti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 5 antibodyAnti-KMT2E antibodyAnti-MLL5 antibody |
Information sourced from Uniprot.org