Anti-KLHL3 antibody (1-110) (STJ115716)

Name: Anti-KLHL3 antibody (1-110) (STJ115716)
SKU: STJ115716
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

ClonalityPolyclonal

HostRabbit

ConjugationUnconjugated

IsotypeIgG

STJ115716

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Short Description	Rabbit polyclonal KLHL3 (1-110) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
Applications	WB/ELISA
Host	Rabbit
Reactivity	Human/Mouse
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Clonality	Polyclonal
Isotype	IgG
Conjugation	Unconjugated
Concentration	Lot specific
Purification	Affinity purification
Dilution Range	WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation	PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage Instruction	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol	KLHL3
Gene ID	26249
Uniprot ID	KLHL3_HUMAN
Immunogen Region	1-110
Immunogen Sequence	MEGESVKLSSQTLIQAGDDE KNQRTITVNPAHMGKAFKVM NELRSKQLLCDVMIVAEDVE IEAHRVVLAACSPYFCAMFT GDMSESKAKKIEIKDVDGQT LSKLIDYIYT
Specificity	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human KLHL3 (NP_059111.2).

Tissue Specificity	Widely expressed.
Post Translational Modifications	Phosphorylation at Ser-433 by PKA or PKC decreases the interaction with WNK1 and WNK4, leading to inhibit their degradation by the BCR(KLHL3) complex. Phosphorylated at Ser-433 by PKC in response to angiotensin II signaling, decreasing ability to promote degradation of WNK1 and WNK4, leading to activation of Na-Cl cotransporter SLC12A3/NCC. Phosphorylation at Ser-433 is increased by insulin. Dephosphorylated at Ser-433 by calcineurin PPP3CA, promoting degradation of WNK1 and WNK4.
Function	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption. The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3. The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation.
Protein Name	Kelch-Like Protein 3
Database Links	Reactome: R-HSA-8951664 Reactome: R-HSA-983168
Cellular Localisation	Cytoplasm Cytosol Cytoskeleton
Alternative Antibody Names	Anti-Kelch-Like Protein 3 antibody Anti-KLHL3 antibody Anti-KIAA1129 antibody

Information sourced from Uniprot.org