| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylation at Ser-433 by PKA or PKC decreases the interaction with WNK1 and WNK4, leading to inhibit their degradation by the BCR(KLHL3) complex. Phosphorylated at Ser-433 by PKC in response to angiotensin II signaling, decreasing ability to promote degradation of WNK1 and WNK4, leading to activation of Na-Cl cotransporter SLC12A3/NCC. Phosphorylation at Ser-433 is increased by insulin. Dephosphorylated at Ser-433 by calcineurin PPP3CA, promoting degradation of WNK1 and WNK4. |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption. The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3. The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation. |
| Protein Name | Kelch-Like Protein 3 |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytosolCytoskeleton |
| Alternative Antibody Names | Anti-Kelch-Like Protein 3 antibodyAnti-KLHL3 antibodyAnti-KIAA1129 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TXNDC9 knockout (KO) HeLa cells, using [KO Validated] TXNDC9 Rabbit polyclonal antibody (STJ11100745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89683/358174/STJ11100745_1__65395.1713122481.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TXNDC9 knockout (KO) HeLa cells, using [KO Validated] TXNDC9 Rabbit polyclonal antibody (STJ11100745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
