• Immunofluorescence analysis of Mouse-colon tissue. 1, Kif 7 monoclonal antibody (3F8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • IHC staining of Mouse Kidney tissue, diluted at 1:200.
  • Immunofluorescence analysis of Hela cell. 1, C/EBP Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Kif 7 monoclonal antibody (3F8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-KIF7 antibody [3F8] (STJ96965)

SKU:
STJ96965

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Kinesin-like protein KIF7 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 3F8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 500-2000
1:200
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF7
Gene ID: 374654
Uniprot ID: KIF7_HUMAN
Specificity: The antibody detects endogenous Kif 7 proteins.
Immunogen: Synthetic Peptide of Kif 7
Post Translational Modifications Polyubiquitinated by UBR3.
Function Essential for hedgehog signaling regulation: acts as both a negative and positive regulator of sonic hedgehog (Shh) and Indian hedgehog (Ihh) pathways, acting downstream of SMO, through both SUFU-dependent and -independent mechanisms. Involved in the regulation of microtubular dynamics. Required for proper organization of the ciliary tip and control of ciliary localization of SUFU-GLI2 complexes. Required for localization of GLI3 to cilia in response to Shh. Negatively regulates Shh signaling by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates Shh signaling by preventing the processing of the transcription factor GLI3 into its repressor form. In keratinocytes, promotes the dissociation of SUFU-GLI2 complexes, GLI2 nuclear translocation and Shh signaling activation. Involved in the regulation of epidermal differentiation and chondrocyte development.
Protein Name Kinesin-Like Protein Kif7
Database Links Reactome: R-HSA-5610787
Reactome: R-HSA-5632684
Cellular Localisation Cell Projection
Cilium
Cytoplasm
Cytoskeleton
Cilium Basal Body
Localizes To The Cilium Tip
Alternative Antibody Names Anti-Kinesin-Like Protein Kif7 antibody
Anti-KIF7 antibody
Anti-UNQ340 antibody
Anti-PRO539 antibody

Information sourced from Uniprot.org

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