| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KIF2C (1-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KIF2C |
| Gene ID: |
11004 |
| Uniprot ID: |
KIF2C_HUMAN |
| Immunogen Region: |
1-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human KIF2C/MCAK (NP_006836.2). |
| Immunogen Sequence: |
MAMDSSLQARLFPGLAIKIQ RSNGLIHSANVRTVNLEKSC VSVEWAEGGATKGKEIDFDD VAAINPELLQLLPLHPKDNL PLQENVTIQKQKRRSVNSKI PAPKESLRSRSTRMSTVSEL RITAQENDMEVELPAAANSR KQFSVPPAPTRPSCPAVAEI PLRMVSEEMEEQVHSIRGSS SANPVNSVRR |
| Tissue Specificity | Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary.expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. |
| Post Translational Modifications | Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. Ubiquitinated. |
| Function | In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment. |
| Protein Name | Kinesin-Like Protein Kif2cKinesin-Like Protein 6Mitotic Centromere-Associated KinesinMcak |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2132295Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-6811434Reactome: R-HSA-68877Reactome: R-HSA-9648025Reactome: R-HSA-983189 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusChromosomeCentromereKinetochoreAssociates With The Microtubule Network At The Growing Distal Tip (The Plus-End) Of MicrotubulesProbably Through Interaction With Mtus2/Tip150 And Mapre1Association With Microtubule Plus Ends Is Also Mediated By Interaction With Kif18bCentromeric Localization Requires The Presence Of Bub1 And Sgo2 |
| Alternative Antibody Names | Anti-Kinesin-Like Protein Kif2c antibodyAnti-Kinesin-Like Protein 6 antibodyAnti-Mitotic Centromere-Associated Kinesin antibodyAnti-Mcak antibodyAnti-KIF2C antibodyAnti-KNSL6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96581/379140/STJ117622_1__97810.1713146869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")