• Immunohistochemistry analysis of KIF22 in paraffin-embedded human breast cancer using KIF22 Rabbit polyclonal antibody (STJ117480) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KIF22 in paraffin-embedded human colon carcinoma using KIF22 Rabbit polyclonal antibody (STJ117480) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KIF22 in paraffin-embedded human colon using KIF22 Rabbit polyclonal antibody (STJ117480) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using KIF22 Rabbit polyclonal antibody (STJ117480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-KIF22 antibody (456-665) (STJ117480)

SKU:
STJ117480

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIF22 (456-665) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF22
Gene ID: 3835
Uniprot ID: KIF22_HUMAN
Immunogen Region: 456-665
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 456-665 of human KIF22 (NP_015556.1).
Immunogen Sequence: QGAPLLSTPKRERMVLMKTV EEKDLEIERLKTKQKELEAK MLAQKAEEKENHCPTMLRPL SHRTVTGAKPLKKAVVMPLQ LIQEQAASPNAEIHILKNKG RKRKLESLDALEPEEKAEDC WELQISPELLAHGRQKILDL LNEGSARDLRSLQRIGPKKA QLIVGWRELHGPFSQVEDLE RVEGITGKQMESFLKANILG LAAGQRCGAS
Tissue Specificity Expressed in bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes.
Post Translational Modifications Ubiquitinated.mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Function Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells.
Protein Name Kinesin-Like Protein Kif22
Kinesin-Like Dna-Binding Protein
Kinesin-Like Protein 4
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Kinesin-Like Protein Kif22 antibody
Anti-Kinesin-Like Dna-Binding Protein antibody
Anti-Kinesin-Like Protein 4 antibody
Anti-KIF22 antibody
Anti-KID antibody
Anti-KNSL4 antibody

Information sourced from Uniprot.org

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