Anti-KHSRP antibody (1-180) [S9MR] (STJ11102419)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal KHSRP (1-180) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S9MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KHSRP |
| Gene ID | 8570 |
| Uniprot ID | FUBP2_HUMAN |
| Immunogen Region | 1-180 |
| Immunogen Sequence | MSDYSTGGPPPGPPPPAGGG GGAGGAGGGPPPGPPGAGDR GGGGPGGGGPGGGSAGGPSQ PPGGGGPGIRKDAFADAVQR ARQIAAKIGGDAATTVNNST PDFGFGGQKRQLEDGDQPES KKLASQGDSISSQLGPIHPP PRTSMTEEYRVPDGMVGLII GRGGEQINKIQQDSGCKVQI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human KHSRP (Q92945). |
Additional Info
| Tissue Specificity | Detected in neural and non-neural cell lines. |
| Post Translational Modifications | Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function. |
| Function | Binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs. |
| Protein Name | Far Upstream Element-Binding Protein 2Fuse-Binding Protein 2Kh Type-Splicing Regulatory ProteinKsrpP75 |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-450604 |
| Cellular Localisation | NucleusCytoplasmA Small Proportion Is Also Found In The Cytoplasm Of Neuronal Cell Bodies And Dendrites |
| Alternative Antibody Names | Anti-Far Upstream Element-Binding Protein 2 antibodyAnti-Fuse-Binding Protein 2 antibodyAnti-Kh Type-Splicing Regulatory Protein antibodyAnti-Ksrp antibodyAnti-P75 antibodyAnti-KHSRP antibodyAnti-FUBP2 antibody |
Information sourced from Uniprot.org