• Western blot analysis of extracts of various cell lines, using KHSRP rabbit monoclonal antibody (STJ11102419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded human breast cancer tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded human breast tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded human esophagus tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded human thyroid tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded mouse colon tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded mouse heart tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded mouse liver tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded mouse spleen tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded rat colon tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KHSRP in paraffin-embedded rat liver tissue using KHSRP rabbit monoclonal antibody (STJ11102419) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of HepG2 cells using 3 Mu g KHSRP antibody (STJ11102419). Western blot was performed from the immunoprecipitate using KHSRP antibody (STJ11102419) at a dilution of 1:1000.

Anti-KHSRP antibody (1-180) [S9MR] (STJ11102419)

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STJ11102419

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-KHSRP (1-180) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KHSRP
Gene ID: 8570
Uniprot ID: FUBP2_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human KHSRP (Q92945).
Immunogen Sequence: MSDYSTGGPPPGPPPPAGGG GGAGGAGGGPPPGPPGAGDR GGGGPGGGGPGGGSAGGPSQ PPGGGGPGIRKDAFADAVQR ARQIAAKIGGDAATTVNNST PDFGFGGQKRQLEDGDQPES KKLASQGDSISSQLGPIHPP PRTSMTEEYRVPDGMVGLII GRGGEQINKIQQDSGCKVQI
Tissue Specificity Detected in neural and non-neural cell lines.
Post Translational Modifications Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.
Function Binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Protein Name Far Upstream Element-Binding Protein 2
Fuse-Binding Protein 2
Kh Type-Splicing Regulatory Protein
Ksrp
P75
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-450604
Cellular Localisation Nucleus
Cytoplasm
A Small Proportion Is Also Found In The Cytoplasm Of Neuronal Cell Bodies And Dendrites
Alternative Antibody Names Anti-Far Upstream Element-Binding Protein 2 antibody
Anti-Fuse-Binding Protein 2 antibody
Anti-Kh Type-Splicing Regulatory Protein antibody
Anti-Ksrp antibody
Anti-P75 antibody
Anti-KHSRP antibody
Anti-FUBP2 antibody

Information sourced from Uniprot.org

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