• Confocal imaging of U-2 OS cells using KHDRBS1/Sam68 Rabbit monoclonal antibody (STJ11101963, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using KHDRBS1/Sam68 Rabbit monoclonal antibody (STJ11101963, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of C6 cells using KHDRBS1/Sam68 Rabbit monoclonal antibody (STJ11101963, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using KHDRBS1/KHDRBS1/Sam68 Rabbit monoclonal antibody (STJ11101963) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using KHDRBS1/KHDRBS1/Sam68 Rabbit monoclonal antibody (STJ11101963) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using KHDRBS1/KHDRBS1/Sam68 Rabbit monoclonal antibody (STJ11101963) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using KHDRBS1/KHDRBS1/Sam68 Rabbit monoclonal antibody (STJ11101963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KHDRBS1 antibody (1-100) [S3MR] (STJ11101963)

SKU:
STJ11101963

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SAM68 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KHDRBS1
Gene ID: 10657
Uniprot ID: KHDR1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KHDRBS1 (Q07666).
Immunogen Sequence: MQRRDDPAARMSRSSGRSGS MDPSGAHPSVRQTPSRQPPL PHRSRGGGGGSRGGARASPA TQPPPLLPPSATGPDATVGG PAPTPLLPPSATASVKMEPE
Tissue Specificity Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
Post Translational Modifications Tyrosine phosphorylated by several non-receptor tyrosine kinases including LCK, FYN and JAK3. Also tyrosine phosphorylated by the non-receptor tyrosine kinase SRMS in an EGF-dependent manner. Negatively correlates with ability to bind RNA but required for many interactions with proteins. Phosphorylation by PTK6 negatively regulates its RNA binding ability. Phosphorylation by PTK6 at Tyr-440 dictates the nuclear localization of KHDRBS1. Phosphorylation at Tyr-387 disrupts interaction with APC. Phosphorylation at tyrosine residues by FYN inverts activity on modulation of BCL2L1 alternative splicing. Acetylated. Positively correlates with ability to bind RNA. Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 and FNBP4/FBP30.
Function Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to. RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-AUUAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner. In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1. Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4. Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
Protein Name Kh Domain-Containing - Rna-Binding - Signal Transduction-Associated Protein 1
Gap-Associated Tyrosine Phosphoprotein P62
Src-Associated In Mitosis 68 Kda Protein
Sam68
P21 Ras Gtpase-Activating Protein-Associated P62
P68
Database Links Reactome: R-HSA-8849468
Cellular Localisation Nucleus
Cytoplasm
Membrane
Predominantly Located In The Nucleus But Also Located Partially In The Cytoplasm
Alternative Antibody Names Anti-Kh Domain-Containing - Rna-Binding - Signal Transduction-Associated Protein 1 antibody
Anti-Gap-Associated Tyrosine Phosphoprotein P62 antibody
Anti-Src-Associated In Mitosis 68 Kda Protein antibody
Anti-Sam68 antibody
Anti-P21 Ras Gtpase-Activating Protein-Associated P62 antibody
Anti-P68 antibody
Anti-KHDRBS1 antibody
Anti-SAM68 antibody

Information sourced from Uniprot.org

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