• Western blot analysis of extracts of various cell lines, using KCNS3 antibody (STJ110215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using KCNS3 antibody (STJ110215) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using KCNS3 antibody (STJ110215) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using KCNS3 antibody (STJ110215) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using KCNS3 antibody (STJ110215) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using KCNS3 antibody (STJ110215) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using KCNS3 antibody (STJ110215) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using KCNS3 antibody (STJ110215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KCNS3 antibody (1-180) (STJ110215)

SKU:
STJ110215

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KCNS3 (1-180) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNS3
Gene ID: 3790
Uniprot ID: KCNS3_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human KCNS3 (NP_002243.3).
Immunogen Sequence: MVFGEFFHRPGQDEELVNLN VGGFKQSVDQSTLLRFPHTR LGKLLTCHSEEAILELCDDY SVADKEYYFDRNPSLFRYVL NFYYTGKLHVMEELCVFSFC QEIEYWGINELFIDSCCSNR YQERKEENHEKDWDQKSHDV STDSSFEESSLFEKELEKFD TLRFGQLRKKIWIRMENPAY
Tissue Specificity Detected in whole normal term placental and placental chorionic plate arteries and veins. Detected in syncytiotrophoblast and in blood vessel endothelium within intermediate villi and chorionic plate (at protein level). Detected in most tissues, but not in peripheral blood lymphocytes. The highest levels of expression are in lung.
Function Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1.modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1. Heterotetrameric channel activity formed with KCNB1 show increased current amplitude with the threshold for action potential activation shifted towards more negative values in hypoxic-treated pulmonary artery smooth muscle cells.
Protein Name Potassium Voltage-Gated Channel Subfamily S Member 3
Delayed-Rectifier K(+ Channel Alpha Subunit 3
Voltage-Gated Potassium Channel Subunit Kv9.3
Database Links Reactome: R-HSA-1296072
Reactome: R-HSA-381676
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
May Not Reach The Plasma Membrane But Remain In An Intracellular Compartment In The Absence Of Kcnb1
Alternative Antibody Names Anti-Potassium Voltage-Gated Channel Subfamily S Member 3 antibody
Anti-Delayed-Rectifier K(+ Channel Alpha Subunit 3 antibody
Anti-Voltage-Gated Potassium Channel Subunit Kv9.3 antibody
Anti-KCNS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance