• Immunohistochemistry analysis of KCNJ15 in paraffin-embedded mouse kidney using KCNJ15 Rabbit polyclonal antibody (STJ117477) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KCNJ15 in paraffin-embedded human breast cancer using KCNJ15 Rabbit polyclonal antibody (STJ117477) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of KCNJ15 in paraffin-embedded rat heart using KCNJ15 Rabbit polyclonal antibody (STJ117477) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using KCNJ15 Rabbit polyclonal antibody (STJ117477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KCNJ15 antibody (221-375) (STJ117477)

SKU:
STJ117477

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KCNJ15 (221-375) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNJ15
Gene ID: 3772
Uniprot ID: KCJ15_HUMAN
Immunogen Region: 221-375
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 221-375 of human KCNJ15 (NP_002234.2).
Immunogen Sequence: VTKEGERILLNQATVKFHVD SSSESPFLILPMTFYHVLDE TSPLRDLTPQNLKEKEFELV VLLNATVESTSAVCQSRTSY IPEEIYWGFEFVPVVSLSKN GKYVADFSQFEQIRKSPDCT FYCADSEKQQLEEKYRQEDQ RERELRTLLLQQSNV
Function Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium.as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Protein Name Atp-Sensitive Inward Rectifier Potassium Channel 15
Inward Rectifier K(+ Channel Kir1.3
Inward Rectifier K(+ Channel Kir4.2
Potassium Channel - Inwardly Rectifying Subfamily J Member 15
Database Links Reactome: R-HSA-1296041
Reactome: R-HSA-997272
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Sensitive Inward Rectifier Potassium Channel 15 antibody
Anti-Inward Rectifier K(+ Channel Kir1.3 antibody
Anti-Inward Rectifier K(+ Channel Kir4.2 antibody
Anti-Potassium Channel - Inwardly Rectifying Subfamily J Member 15 antibody
Anti-KCNJ15 antibody
Anti-KCNJ14 antibody

Information sourced from Uniprot.org

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