Anti-KCC3/SLC12A6, Biotinylated antibody (Internal) {Biotin} (STJ73234)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationBiotin
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-KCC3/SLC12A6, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 120kDa band observed in Human Heart lysates (calculated MW ranging 121-128kDakDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035 |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC12A6 |
| Gene ID | 9990 |
| Uniprot ID | S12A6_HUMAN |
| Accession Number | NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | SRHIDVCSKTKE. |
| Specificity | This antibody is expected to recognise all reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1) ; Reported variants represent identicial protein: NP_001035959.1; NP_001035960.1 |
Additional Info
| Post Translational Modifications | Phosphorylated, phosphorylation regulates transporter activity. Phosphorylated at Thr-991 and Thr-1048 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. N-glycosylated. |
| Function | Isoform 1: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. Isoform 2: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 3: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 4: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 5: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 6: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). |
| Protein Name | Solute Carrier Family 12 Member 6Electroneutral Potassium-Chloride Cotransporter 3K-Cl Cotransporter 3 |
| Database Links | Reactome: R-HSA-426117Reactome: R-HSA-5619039 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell Membrane |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 6 antibodyAnti-Electroneutral Potassium-Chloride Cotransporter 3 antibodyAnti-K-Cl Cotransporter 3 antibodyAnti-SLC12A6 antibodyAnti-KCC3 antibody |
Information sourced from Uniprot.org