Biotinylated STJ71334 (0. 1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-KCC3/SLC12A6, Biotinylated antibody (Internal) {Biotin} (STJ73234)

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STJ73234-100
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-KCC3/SLC12A6, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:16000.
WB: Approx 120kDa band observed in Human Heart lysates (calculated MW ranging 121-128kDakDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLC12A6
Gene ID: 9990
Uniprot ID: S12A6_HUMAN
Immunogen Region: Internal
Accession Number: NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1
Specificity: This antibody is expected to recognise all reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1) ; Reported variants represent identicial protein: NP_001035959.1; NP_001035960.1
Immunogen Sequence: SRHIDVCSKTKE.
Post Translational Modifications Phosphorylated, phosphorylation regulates transporter activity. Phosphorylated at Thr-991 and Thr-1048 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. N-glycosylated.
Function Isoform 1: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. Isoform 2: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 3: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 4: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 5: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 6: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable).
Protein Name Solute Carrier Family 12 Member 6
Electroneutral Potassium-Chloride Cotransporter 3
K-Cl Cotransporter 3
Database Links Reactome: R-HSA-426117
Reactome: R-HSA-5619039
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 6 antibody
Anti-Electroneutral Potassium-Chloride Cotransporter 3 antibody
Anti-K-Cl Cotransporter 3 antibody
Anti-SLC12A6 antibody
Anti-KCC3 antibody

Information sourced from Uniprot.org

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