• Immunoprecipitation analysis of 300 Mu g extracts of A-549 cells using 3 Mu g Kaiso/ZBTB33 antibody (STJ11103534). Western blot was performed from the immunoprecipitate using Kaiso/ZBTB33 antibody (STJ11103534) at a dilution of 1:1000.
  • Immunofluorescence analysis of HeLa cells using Kaiso/ZBTB33 Rabbit monoclonal antibody (STJ11103534) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Kaiso/ZBTB33 Rabbit monoclonal antibody (STJ11103534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Kaiso/ZBTB33 antibody (573-672) [S4MR] (STJ11103534)

SKU:
STJ11103534

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Kaiso/ZBTB33 (573-672) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZBTB33
Gene ID: 10009
Uniprot ID: KAISO_HUMAN
Immunogen Region: 573-672
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 573-672 of human Kaiso/ZBTB33 (Q86T24).
Immunogen Sequence: HSQDPSGDSKLYRLHPCRSL QIRQYAYLSDRSSTIPAMKD DGIGYKVDTGKEPPVGTTTS TQNKPMTWEDIFIQQENDSI FKQNVTDGSTEFEFIIPESY
Tissue Specificity Expressed in vascular endothelium.
Function Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1.
Protein Name Transcriptional Regulator Kaiso
Zinc Finger And Btb Domain-Containing Protein 33
Cellular Localisation Nucleus
Cytoplasm
Also Cytoplasmic In Cells Grown At High Densities
Alternative Antibody Names Anti-Transcriptional Regulator Kaiso antibody
Anti-Zinc Finger And Btb Domain-Containing Protein 33 antibody
Anti-ZBTB33 antibody
Anti-KAISO antibody
Anti-ZNF348 antibody

Information sourced from Uniprot.org

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