• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g JunD antibody (STJ11103587). Western blot was performed from the immunoprecipitate using JunD antibody (STJ11103587) at a dilution of 1:1000.
  • Immunohistochemistry analysis of JunD in paraffin-embedded mouse colon tissue using JunD Rabbit monoclonal antibody (STJ11103587) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JunD in paraffin-embedded rat colon tissue using JunD Rabbit monoclonal antibody (STJ11103587) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JunD in paraffin-embedded human small intestine tissue using JunD Rabbit monoclonal antibody (STJ11103587) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JunD in paraffin-embedded human thyroid tissue using JunD Rabbit monoclonal antibody (STJ11103587) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of JunD in paraffin-embedded human esophagus tissue using JunD Rabbit monoclonal antibody (STJ11103587) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using JunD Rabbit monoclonal antibody (STJ11103587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-JunD antibody (1-141) [S7MR] (STJ11103587)

SKU:
STJ11103587

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-JunD (1-141) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUND
Gene ID: 3727
Uniprot ID: JUND_HUMAN
Immunogen Region: 1-141
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human JunD (P17535).
Immunogen Sequence: METPFYGDEALSGLGGGASG SGGSFASPGRLFPGAPPTAA AGSMMKKDALTLSLSEQVAA ALKPAAAPPPTPLRADGAPS AAPPDGLLASPDLGLLKLAS PELERLIIQSNGLVTTTPTS SQFLYPKVAASEEQEFAEGF V
Post Translational Modifications Phosphorylated by MAP kinases MAPK8 and MAPK10.phosphorylation is inhibited in the presence of MEN1.
Function Transcription factor binding AP-1 sites. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGAGCTCA-5' and enhancing their transcriptional activity.
Protein Name Transcription Factor Jund
Transcription Factor Ap-1 Subunit Jund
Database Links Reactome: R-HSA-9018519
Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Jund antibody
Anti-Transcription Factor Ap-1 Subunit Jund antibody
Anti-JUND antibody

Information sourced from Uniprot.org

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