• Western blot analysis of lysates from HL-60 cells, using IRF8 Rabbit polyclonal antibody (STJ115632) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IRF8 antibody (137-426) (STJ115632)

SKU:
STJ115632

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IRF8 (137-426) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRF8
Gene ID: 3394
Uniprot ID: IRF8_HUMAN
Immunogen Region: 137-426
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 137-426 of human IRF8 (NP_002154.1).
Immunogen Sequence: ECGRSEIDELIKEPSVDDYM GMIKRSPSPPEACRSQLLPD WWAQQPSTGVPLVTGYTTYD AHHSAFSQMVISFYYGGKLV GQATTTCPEGCRLSLSQPGL PGTKLYGPEGLELVRFPPAD AIPSERQRQVTRKLFGHLER GVLLHSSRQGVFVKRLCQGR VFCSGNAVVCKGRPNKLERD EVVQVFDTSQFFRELQQFYN SQGRLPDGRVVLCFGEEFPD MAPLRSKLILVQIEQLYVR
Tissue Specificity Predominantly expressed in lymphoid tissues.
Post Translational Modifications Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes. Ubiquitination leads to its degradation by the proteasome. Sumoylated with SUMO3. Desumoylated by SENP1.
Function Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor. Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection. Positively regulates macroautophagy in dendritic cells. Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1.
Protein Name Interferon Regulatory Factor 8
Irf-8
Interferon Consensus Sequence-Binding Protein
H-Icsbp
Icsbp
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-909733
Cellular Localisation Nucleus
Cytoplasm
In Resting Macrophages
Localizes In The Cytoplasm
Translocated In The Nucleus Upon Ifn-Gamma Induction
Alternative Antibody Names Anti-Interferon Regulatory Factor 8 antibody
Anti-Irf-8 antibody
Anti-Interferon Consensus Sequence-Binding Protein antibody
Anti-H-Icsbp antibody
Anti-Icsbp antibody
Anti-IRF8 antibody
Anti-ICSBP1 antibody

Information sourced from Uniprot.org

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