| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ITGA6 |
| Gene ID: | 3655 |
| Uniprot ID: | ITA6_HUMAN |
| Immunogen Region: | 481-591 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 481-591 of human Integrin alpha 6 (ITGA6/CD49f) (NP_001073286.1). |
| Immunogen Sequence: | IDLRQKTACGAPSGICLQVK SCFEYTANPAGYNPSISIVG TLEAEKERRKSGLSSRVQFR NQGSEPKYTQELTLKRQKQK VCMEETLWLQDNIRDKLRPI PITASVEIQEP |
| Tissue Specificity | Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines.they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Processed integrin alpha-6: Expressed at low levels in normal prostate tissue with elevated levels in prostate cancer tissue (at protein level). |
| Post Translational Modifications | Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand. Undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner to produce processed integrin alpha-6 (alpha6p). Production of alpha6p enhances prostate cancer cell invasion and migration. Palmitoylation by DHHC3 enhances stability and cell surface expression. |
| Function | Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling. |
| Protein Name | Integrin Alpha-6 Cd49 Antigen-Like Family Member F Vla-6 Cd Antigen Cd49f Cleaved Into - Integrin Alpha-6 Heavy Chain - Integrin Alpha-6 Light Chain - Processed Integrin Alpha-6 Alpha6p |
| Database Links | Reactome: R-HSA-2022090 Reactome: R-HSA-210991 Reactome: R-HSA-216083 Reactome: R-HSA-3000157 Reactome: R-HSA-3000170 Reactome: R-HSA-446107 Reactome: R-HSA-9725554 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Lipid-Anchor |
| Alternative Antibody Names | Anti-Integrin Alpha-6 antibody Anti-Cd49 Antigen-Like Family Member F antibody Anti-Vla-6 antibody Anti-Cd Antigen Cd49f Cleaved Into - Integrin Alpha-6 Heavy Chain - Integrin Alpha-6 Light Chain - Processed Integrin Alpha-6 antibody Anti-Alpha6p antibody Anti-ITGA6 antibody |
Information sourced from Uniprot.org