• Western blot analysis of various lysates, using IMPDH2 antibody (STJ11102455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts from wild type (WT) and IMPDH2 knockout (KO) NIH/3T3 (KO) cells, using IMPDH2 antibody (STJ11102455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of C6 cells using IMPDH2 rabbit monoclonal antibody (STJ11102455) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using IMPDH2 rabbit monoclonal antibody (STJ11102455) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using IMPDH2 rabbit monoclonal antibody (STJ11102455) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IMPDH2 antibody (100-300) [S5MR] (STJ11102455)

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STJ11102455

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IMPDH2 (100-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IMPDH2
Gene ID: 3615
Uniprot ID: IMDH2_HUMAN
Immunogen Region: 100-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human IMPDH2 (P12268).
Immunogen Sequence: QANEVRKVKKYEQGFITDPV VLSPKDRVRDVFEAKARHGF CGIPITDTGRMGSRLVGIIS SRDIDFLKEEEHDCFLEEIM TKREDLVVAPAGITLKEANE ILQRSKKGKLPIVNEDDELV AIIARTDLKKNRDYPLASKD AKKQLLCGAAIGTHEDDKYR LDLLAQAGVDVVVLDSSQGN SIFQINMIKYIKDKYPNLQV I
Tissue Specificity IMPDH1 is the main species in normal leukocytes and IMPDH2 predominates over IMPDH1 in the tumor.
Post Translational Modifications Ubiquitinated leading to its degradation by the proteasome. The N-terminus is blocked. Acetylated by CLOCK in a circadian manner.
Function Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
Protein Name Inosine-5'-Monophosphate Dehydrogenase 2
Imp Dehydrogenase 2
Impd 2
Impdh 2
Inosine-5'-Monophosphate Dehydrogenase Type Ii
Imp Dehydrogenase Ii
Impdh-Ii
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-73817
Reactome: R-HSA-9679191
Reactome: R-HSA-9748787
Cellular Localisation Cytoplasm
Nucleus
Cytosol
Can Form Fiber-Like Subcellular Structures Termed 'Cytoophidia' In Response To Intracellular Guanine-Nucleotide Depletion
Alternative Antibody Names Anti-Inosine-5'-Monophosphate Dehydrogenase 2 antibody
Anti-Imp Dehydrogenase 2 antibody
Anti-Impd 2 antibody
Anti-Impdh 2 antibody
Anti-Inosine-5'-Monophosphate Dehydrogenase Type Ii antibody
Anti-Imp Dehydrogenase Ii antibody
Anti-Impdh-Ii antibody
Anti-IMPDH2 antibody
Anti-IMPD2 antibody

Information sourced from Uniprot.org

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