| Tissue Specificity | IMPDH1 is the main species in normal leukocytes and IMPDH2 predominates over IMPDH1 in the tumor. |
| Post Translational Modifications | Ubiquitinated leading to its degradation by the proteasome. The N-terminus is blocked. Acetylated by CLOCK in a circadian manner. |
| Function | Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. |
| Protein Name | Inosine-5'-Monophosphate Dehydrogenase 2Imp Dehydrogenase 2Impd 2Impdh 2Inosine-5'-Monophosphate Dehydrogenase Type IiImp Dehydrogenase IiImpdh-Ii |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-73817Reactome: R-HSA-9679191Reactome: R-HSA-9748787 |
| Cellular Localisation | CytoplasmNucleusCytosolCan Form Fiber-Like Subcellular Structures Termed 'Cytoophidia' In Response To Intracellular Guanine-Nucleotide Depletion |
| Alternative Antibody Names | Anti-Inosine-5'-Monophosphate Dehydrogenase 2 antibodyAnti-Imp Dehydrogenase 2 antibodyAnti-Impd 2 antibodyAnti-Impdh 2 antibodyAnti-Inosine-5'-Monophosphate Dehydrogenase Type Ii antibodyAnti-Imp Dehydrogenase Ii antibodyAnti-Impdh-Ii antibodyAnti-IMPDH2 antibodyAnti-IMPD2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates, using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91372/406336/STJ11102455_1__37446.1744892217.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.")
![Western blot analysis of lysates from wild type (WT) and IMPDH2 knockout (KO) NIH/3T3 (KO) cells, using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91372/406337/STJ11102455_2__42737.1744892217.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IMPDH2 knockout (KO) NIH/3T3 (KO) cells, using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.")
![Confocal imaging of NIH/3T3 cells using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91372/406338/STJ11102455_3__18975.1744892218.jpg?c=1 "Confocal imaging of NIH/3T3 cells using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Confocal imaging of C6 cells using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91372/406339/STJ11102455_4__93737.1744892219.jpg?c=1 "Confocal imaging of C6 cells using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Confocal imaging of C2C12 cells using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91372/406340/STJ11102455_5__78044.1744892219.jpg?c=1 "Confocal imaging of C2C12 cells using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Western blot analysis of various lysates, using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/91372/406336/STJ11102455_1__37446.1744892217.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] IMPDH2 Rabbit mAb (STJ11102455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.")
