• Western blot analysis of extracts of various cell lines, using IL25 antibody (STJ110551) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.
  • Immunofluorescence analysis of paraffin-embedded mouse lung using IL25 Rabbit polyclonal antibody (STJ110551) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human lung cancer cells using IL25 antibody (STJ110551) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat bronchus using IL25 Rabbit polyclonal antibody (STJ110551) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat bronchus cells using IL25 antibody (STJ110551) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded human lung cancer using IL25 Rabbit polyclonal antibody (STJ110551) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse lung cells using IL25 antibody (STJ110551) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with 17E/IL25 using IL25 Rabbit polyclonal antibody (STJ110551) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL25 antibody (33-177) (STJ110551)

SKU:
STJ110551

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL25 (33-177) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL25
Gene ID: 64806
Uniprot ID: IL25_HUMAN
Immunogen Region: 33-177
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).
Immunogen Sequence: YSHWPSCCPSKGQDTSEELL RWSTVPVPPLEPARPNRHPE SCRASEDGPLNSRAISPWRY ELDRDLNRLPQDLYHARCLC PHCVSLQTGSHMDPRGNSEL LYHNQTVFYRRPCHGEKGTH KGYCLERRLYRVSLACVCVR PRVMG
Tissue Specificity Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
Function Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways. Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13.
Protein Name Interleukin-25
Il-25
Interleukin-17e
Il-17e
Database Links Reactome: R-HSA-448424
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-25 antibody
Anti-Il-25 antibody
Anti-Interleukin-17e antibody
Anti-Il-17e antibody
Anti-IL25 antibody
Anti-IL17E antibody
Anti-UNQ3120 antibody
Anti-PRO10272 antibody

Information sourced from Uniprot.org

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