• Western blot analysis of HL-60, using IL22 antibody (STJ27972) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of HL-60 cells using IL22 rabbit polyclonal antibody (STJ27972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Raji cells using IL22 rabbit polyclonal antibody (STJ27972) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL22 antibody (34-179) (STJ27972)

SKU:
STJ27972

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL22 (34-179) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL22
Gene ID: 50616
Uniprot ID: IL22_HUMAN
Immunogen Region: 34-179
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-179 of human IL22 (NP_065386.1).
Immunogen Sequence: APISSHCRLDKSNFQQPYIT NRTFMLAKEASLADNNTDVR LIGEKLFHGVSMSERCYLMK QVLNFTLEEVLFPQSDRFQP YMQEVVPFLARLSNRLSTCH IEGDDLHIQRNVQKLKDTVK KLGESGEIKAIGELDLLFMS LRNACI
Function Cytokine that plays a critical role in modulating tissue responses during inflammation. Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage. Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN. Promotes epithelial cell spreading.
Protein Name Interleukin-22
Il-22
Cytokine Zcyto18
Il-10-Related T-Cell-Derived-Inducible Factor
Il-Tif
Database Links Reactome: R-HSA-8854691
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interleukin-22 antibody
Anti-Il-22 antibody
Anti-Cytokine Zcyto18 antibody
Anti-Il-10-Related T-Cell-Derived-Inducible Factor antibody
Anti-Il-Tif antibody
Anti-IL22 antibody
Anti-ILTIF antibody
Anti-ZCYTO18 antibody
Anti-UNQ3099 antibody
Anti-PRO10096 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance