• Western blot analysis of extracts of Raji cells, using IKZF3 antibody (STJ11103626) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunoprecipitation analysis of 300 Mu g extracts of Raji cells using 3 Mu g IKZF3 antibody (STJ11103626). Western blot was performed from the immunoprecipitate using IKZF3 antibody (STJ11103626) at a dilution of 1:500.
  • Western blot analysis of extracts of various cell lines, using IKZF3 antibody (STJ11103626) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of paraffin-embedded rat spleen using IKZF3 Rabbit monoclonal antibody (STJ11103626) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat lung, using IKZF3 antibody (STJ11103626) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of IKZF3 in paraffin-embedded rat spleen tissue using IKZF3 Rabbit monoclonal antibody (STJ11103626) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using IKZF3 antibody (STJ11103626) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IKZF3 in paraffin-embedded mouse spleen tissue using IKZF3 Rabbit monoclonal antibody (STJ11103626) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human tonsil using IKZF3 antibody (STJ11103626) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IKZF3 in paraffin-embedded human spleen tissue using IKZF3 Rabbit monoclonal antibody (STJ11103626) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse spleen using IKZF3 antibody (STJ11103626) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat lung, using IKZF3 Rabbit monoclonal antibody (STJ11103626) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat lung using IKZF3 antibody (STJ11103626) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using IKZF3 Rabbit monoclonal antibody (STJ11103626) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry of paraffin-embedded rat spleen using IKZF3 antibody (STJ11103626) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Raji cells, using IKZF3 Rabbit monoclonal antibody (STJ11103626) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of rat spleen using IKZF3 rabbit monoclonal antibody (STJ11103626) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen using IKZF3 rabbit monoclonal antibody (STJ11103626) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IKZF3 antibody (400-509) [S6MR] (STJ11103626)

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STJ11103626

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IKZF3 (400-509) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IKZF3
Gene ID: 22806
Uniprot ID: IKZF3_HUMAN
Immunogen Region: 400-509
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-509 of human IKZF3 (Q9UKT9).
Immunogen Sequence: IYQQNHMVLSRARNGMPLLK EVPRSYELLKPPPICPRDSV KVINKEGEVMDVYRCDHCRV LFLDYVMFTIHMGCHGFRDP FECNMCGYRSHDRYEFSSHI ARGEHRALLK
Tissue Specificity Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
Post Translational Modifications Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
Function Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
Protein Name Zinc Finger Protein Aiolos
Ikaros Family Zinc Finger Protein 3
Cellular Localisation Nucleus
Cytoplasm
Isoform 1: Nucleus
Isoform 3: Nucleus
Isoform 11: Nucleus
Isoform 14: Nucleus
Isoform 12: Cytoplasm
Alternative Antibody Names Anti-Zinc Finger Protein Aiolos antibody
Anti-Ikaros Family Zinc Finger Protein 3 antibody
Anti-IKZF3 antibody
Anti-ZNFN1A3 antibody

Information sourced from Uniprot.org

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