• Confocal imaging of HeLa cells using IKK Beta Rabbit monoclonal antibody (STJ11101726, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-IKBKB antibody (450-756) [S6MR] (STJ11101726)

SKU:
STJ11101726

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Host: Rabbit
Applications: IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IKK Beta (450-756) is suitable for use in Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IKBKB
Gene ID: 3551
Uniprot ID: IKKB_HUMAN
Immunogen Region: 450-756
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 450-756 of human IKK Beta (O14920).
Immunogen Sequence: GQRAAMMNLLRNNSCLSKMK NSMASMSQQLKAKLDFFKTS IQIDLEKYSEQTEFGITSDK LLLAWREMEQAVELCGRENE VKLLVERMMALQTDIVDLQR SPMGRKQGGTLDDLEEQARE LYRRLREKPRDQRTEGDSQE MVRLLLQAIQSFEKKVRVIY TQLSKTVVCKQKALELLPKV EEVVSLMNEDEKTVVRLQEK RQKELWNLLKIACSKVRGPV SGSPDSMNASRLSQPGQLM
Tissue Specificity Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Post Translational Modifications Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ.which enhances activity. Phosphorylated by MAP3K7/TAK1 in response to NOD1 and NOD2 signaling, promoting activation and phosphorylation of NF-kappa-B inhibitors, leading to NF-kappa-B activation. Once activated, autophosphorylates on the C-terminal serine cluster.which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B. (Microbial infection) Acetylation of Thr-180 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway. Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. 'Ser-163' does not serve as a monoubiquitination site. ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. (Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia YopJ. Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B.
Function Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus.
Protein Name Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta
I-Kappa-B-Kinase Beta
Ikk-B
Ikk-Beta
Ikbkb
I-Kappa-B Kinase 2
Ikk-2
Ikk2
Nuclear Factor Nf-Kappa-B Inhibitor Kinase Beta
Nfkbikb
Serine/Threonine Protein Kinase Ikbkb
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1236974
Reactome: R-HSA-168638
Reactome: R-HSA-168927
Reactome: R-HSA-1810476
Reactome: R-HSA-202424
Reactome: R-HSA-209543
Reactome: R-HSA-209560
Reactome: R-HSA-2871837
Reactome: R-HSA-445989
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5602636
Reactome: R-HSA-5603027
Reactome: R-HSA-5603029
Reactome: R-HSA-5607764
Reactome: R-HSA-5684264
Reactome: R-HSA-9020702
Reactome: R-HSA-933542
Reactome: R-HSA-933543
Reactome: R-HSA-937039
Reactome: R-HSA-937041
Reactome: R-HSA-9705671
Reactome: R-HSA-975144
Reactome: R-HSA-9758274
Cellular Localisation Cytoplasm
Nucleus
Membrane Raft
Colocalized With Dpp4 In Membrane Rafts
Alternative Antibody Names Anti-Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta antibody
Anti-I-Kappa-B-Kinase Beta antibody
Anti-Ikk-B antibody
Anti-Ikk-Beta antibody
Anti-Ikbkb antibody
Anti-I-Kappa-B Kinase 2 antibody
Anti-Ikk-2 antibody
Anti-Ikk2 antibody
Anti-Nuclear Factor Nf-Kappa-B Inhibitor Kinase Beta antibody
Anti-Nfkbikb antibody
Anti-Serine/Threonine Protein Kinase Ikbkb antibody
Anti-IKBKB antibody
Anti-IKKB antibody

Information sourced from Uniprot.org

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