Anti-IFI35 antibody (1-288) (STJ118812)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IFI35 (1-288) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IFI35 |
| Gene ID | 3430 |
| Uniprot ID | IN35_HUMAN |
| Immunogen Region | 1-288 |
| Immunogen Sequence | MSAPLDAALHALQEEQARLK MRLWDLQQLRKELGDSPKDK VPFSVPKIPLVFRGHTQQDP EVPKSLVSNLRIHCPLLAGS ALITFDDPKVAEQVLQQKEH TINMEECRLRVQVQPLELPM VTTIQVMMSSQLSGRRVLVT GFPASLRLSEEELLDKLEIF FGKTRNGGGDVDVRELLPGS VMLGFARDGVAQRLCQIGQF TVPLGGQQVPLRVSPYVNGE IQKAEIRSQPVPRSVLVLN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human IFI35 (NP_005524.2). |
Additional Info
| Tissue Specificity | Expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. |
| Post Translational Modifications | Phosphorylated. Dephosphorylation correlates with the formation of a complex with NMI. |
| Function | Acts as a signaling pathway regulator involved in innate immune system response. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response.the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation. In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production. In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion. Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines. |
| Protein Name | Interferon-Induced 35 Kda ProteinIfp 35Ifi-35 |
| Database Links | Reactome: R-HSA-909733 |
| Cellular Localisation | CytoplasmNucleusSecretedCytoplasmic Ifi35 Localizes In Punctate Granular StructuresNuclear Localization Increased Is Stimulated By Ifn-AlphaExtracelullar Following Secretion By Macrophage |
| Alternative Antibody Names | Anti-Interferon-Induced 35 Kda Protein antibodyAnti-Ifp 35 antibodyAnti-Ifi-35 antibodyAnti-IFI35 antibodyAnti-IFP35 antibody |
Information sourced from Uniprot.org