| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IFI35 (1-288) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IFI35 |
| Gene ID: |
3430 |
| Uniprot ID: |
IN35_HUMAN |
| Immunogen Region: |
1-288 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human IFI35 (NP_005524.2). |
| Immunogen Sequence: |
MSAPLDAALHALQEEQARLK MRLWDLQQLRKELGDSPKDK VPFSVPKIPLVFRGHTQQDP EVPKSLVSNLRIHCPLLAGS ALITFDDPKVAEQVLQQKEH TINMEECRLRVQVQPLELPM VTTIQVMMSSQLSGRRVLVT GFPASLRLSEEELLDKLEIF FGKTRNGGGDVDVRELLPGS VMLGFARDGVAQRLCQIGQF TVPLGGQQVPLRVSPYVNGE IQKAEIRSQPVPRSVLVLN |
| Tissue Specificity | Expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. |
| Post Translational Modifications | Phosphorylated. Dephosphorylation correlates with the formation of a complex with NMI. |
| Function | Acts as a signaling pathway regulator involved in innate immune system response. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response.the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation. In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production. In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion. Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines. |
| Protein Name | Interferon-Induced 35 Kda ProteinIfp 35Ifi-35 |
| Database Links | Reactome: R-HSA-909733 |
| Cellular Localisation | CytoplasmNucleusSecretedCytoplasmic Ifi35 Localizes In Punctate Granular StructuresNuclear Localization Increased Is Stimulated By Ifn-AlphaExtracelullar Following Secretion By Macrophage |
| Alternative Antibody Names | Anti-Interferon-Induced 35 Kda Protein antibodyAnti-Ifp 35 antibodyAnti-Ifi-35 antibodyAnti-IFI35 antibodyAnti-IFP35 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
