Anti-ICOS/CD278 antibody (21-142 aa) [SRM5417] (STJ11107328)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ICOS/CD278 (21-142 aa) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5417 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Icos |
| Gene ID | 54167 |
| Uniprot ID | ICOS_MOUSE |
| Immunogen Region | 21-142 aa |
| Immunogen Sequence | EINGSADHRMFSFHNGGVQI SCKYPETVQQLKMRLFRERE VLCELTKTKGSGNAVSIKNP MLCLYHLSNNSVSFFLNNPD SSQGSYYFCSLSIFDPPPFQ ERNLSGGYLHIYESQLCCQL KL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 21-142 of mouse ICOS/CD278 (NP_059508.2). |
Additional Info
| Tissue Specificity | Expressed on activated T-cells and resting memory T-cells. High expression seen in the thymic medulla and in the germinal centers and T-cell zones of lymph nodes and Peyer patches. Expressed at low levels in the spleen. |
| Post Translational Modifications | N-glycosylated. |
| Function | Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. |
| Protein Name | Inducible T-Cell CostimulatorActivation-Inducible Lymphocyte Immunomediatory MoleculeCd28 And Ctla-4-Like ProteinCclpCd28-Related Protein 1Crp-1Cd Antigen Cd278 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-6811558Reactome: -MMU-9927354 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Inducible T-Cell Costimulator antibodyAnti-Activation-Inducible Lymphocyte Immunomediatory Molecule antibodyAnti-Cd28 And Ctla-4-Like Protein antibodyAnti-Cclp antibodyAnti-Cd28-Related Protein 1 antibodyAnti-Crp-1 antibodyAnti-Cd Antigen Cd278 antibodyAnti-Icos antibodyAnti-Ailim antibody |
Information sourced from Uniprot.org