• Western blot analysis of various lysates, using IBSP Rabbit polyclonal antibody (STJ11103857) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IBSP antibody (1-100) (STJ11103857)

SKU:
STJ11103857

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IBSP
Gene ID: 3381
Uniprot ID: SIAL_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IBSP (NP_004958.2).
Immunogen Sequence: MKTALILLSILGMACAFSMK NLHRRVKIEDSEENGVFKYR PRYYLYKHAYFYPHLKRFPV QGSSDSSEENGDDSSEEEEE EEETSNEGENNEESNEDEDS
Tissue Specificity Expressed in bone (at protein level). Expressed in trophoblast cells of placenta (at protein level). Expressed in brain.
Post Translational Modifications N-glycosylated.glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains. O-glycosylated at eight sites.mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3).
Protein Name Integrin-Binding Sialoprotein
Bone Sialoprotein 2
Bone Sialoprotein Ii
Bsp Ii
Cell-Binding Sialoprotein
Database Links Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Cellular Localisation Secreted
Alternative Antibody Names Anti-Integrin-Binding Sialoprotein antibody
Anti-Bone Sialoprotein 2 antibody
Anti-Bone Sialoprotein Ii antibody
Anti-Bsp Ii antibody
Anti-Cell-Binding Sialoprotein antibody
Anti-IBSP antibody
Anti-BNSP antibody

Information sourced from Uniprot.org

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