• Confocal imaging of NIH/3T3 cells using HSPA14 Rabbit monoclonal antibody (STJ11101806, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of HSPA14 in paraffin-embedded human colon using HSPA14 Rabbit monoclonal antibody (STJ11101806) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HSPA14 Rabbit monoclonal antibody (STJ11101806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HSPA14 antibody (1-100) [S6MR] (STJ11101806)

SKU:
STJ11101806

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HSPA14 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA14
Gene ID: 51182
Uniprot ID: HSP7E_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSPA14 (Q0VDF9).
Immunogen Sequence: MAAIGVHLGCTSACVAVYKD GRAGVVANDAGDRVTPAVVA YSENEEIVGLAAKQSRIRNI SNTVMKVKQILGRSSSDPQA QKYIAESKCLVIEKNGKLRY
Function Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
Protein Name Heat Shock 70 Kda Protein 14
Hsp70-Like Protein 1
Heat Shock Protein Hsp60
Database Links Reactome: R-HSA-3371453
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Heat Shock 70 Kda Protein 14 antibody
Anti-Hsp70-Like Protein 1 antibody
Anti-Heat Shock Protein Hsp60 antibody
Anti-HSPA14 antibody
Anti-HSP60 antibody
Anti-HSP70L1 antibody

Information sourced from Uniprot.org

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