Confocal imaging of NIH/3T3 cells usingย HSPA14 Rabbit monoclonal antibody (STJ11101806, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HSPA14 antibody (1-100) [S6MR] (STJ11101806)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101806
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General Information

Short DescriptionRabbit monoclonal HSPA14 (1-100) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS6MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolHSPA14
Gene ID51182
Uniprot IDHSP7E_HUMAN
Immunogen Region1-100
Immunogen SequenceMAAIGVHLGCTSACVAVYKD GRAGVVANDAGDRVTPAVVA YSENEEIVGLAAKQSRIRNI SNTVMKVKQILGRSSSDPQA QKYIAESKCLVIEKNGKLRY
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSPA14 (Q0VDF9).

Additional Info

Function Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
Protein Name Heat Shock 70 Kda Protein 14
Hsp70-Like Protein 1
Heat Shock Protein Hsp60
Heat Shock Protein Family A Member 14
Database Links Reactome: R-HSA-3371453
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Heat Shock 70 Kda Protein 14 antibody
Anti-Hsp70-Like Protein 1 antibody
Anti-Heat Shock Protein Hsp60 antibody
Anti-Heat Shock Protein Family A Member 14 antibody
Anti-HSPA14 antibody
Anti-HSP60 antibody
Anti-HSP70L1 antibody

Information sourced from Uniprot.org

Citations

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